AOAC Official Method 2000.03 Ochratoxin A in Barley Immunoaffinity by Column HPLC First Action 2000

(Applicable to the determination of ochratoxin A in barley at >1 ng/g)
See Table 2000.03A for results of the interlaboratory study supporting the acceptance of the method.

A. Principle

Test portion is extracted by blending with acetonitrile-water. The extract is cleaned up by passing through an immunoaffinity column. Ochratoxin A (OTA) is eluted with methanol, further purifed and identified by LC, and quantified by fluorescence.